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Gene Cloning
We are very experienced in gene cloning and protein expression. We have several kinds of expression vectors to assist you to get desired proteins, further more, we can clone and express single gene or hundreds of genes in high-throughput way.
Workflow:
List of services:
Catalog # |
Service
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Turn around time (W)
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Deliverables
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Prices (USD)
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Ag-01
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Cloning (Customer provides template <1.2 kb)
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3-5
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Sequence report; Expression clone; SDS-PAGE
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350
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Ag-02
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Cloning (Customer provides template 1.2-2.0 kb)
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3-5
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Sequence report; Expression clone; SDS-PAGE
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450
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Ag-03
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Cloning (BPI provides template <1.2 kb)
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3-5
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Sequence report; SDS-PAGE
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350
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Ag-04
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Cloning (BPI provides template 1.2-2.0 kb)
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3-5
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Sequence report; SDS-PAGE
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450
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Ag-05
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Cloning (BPI provides template <1.2 kb)
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3-5
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Sequence report; Expression clone; SDS-PAGE
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550
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Ag-06
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Cloning (BPI provides template 1.2-2.0 kb)
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3-5
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Sequence report; Expression clone; SDS-PAGE
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450+1$/5bp
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Ag-07
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Cloning (Gene Synthesis ≤250 bp)
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3-5
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Sequence report; Expression clone; SDS-PAGE
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500
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Ag-08
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Cloning (Gene Synthesis ≤250bp)
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3-5
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Sequence report; Expression clone; SDS-PAGE
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125+1.5$/bp
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Note: BPI-USA provides GST, HIS, or MBP tagged expression vectors. Customer may need to provide other vectors.
Features: High-throughput cloning and expression are available for a large number of genes.
Application: Cloning of target genes (fragments) in new vectors for amplification and expression of proteins.
The information required from customers:
Name of plasmid
Name of the vector
Restriction enzyme recognition site
Selection marker
Amino acid and nucleotide sequences of the inserts
Sequencing data of the recombinant plasmid (insert)
Download order form
Gene Synthesis
Workflow:
List of services:
Catalog # |
Service
|
Turn around time (W)
|
Deliverables
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Price (USD)
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Ag-11
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Gene Synthesis
(≤150bp)
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4-5
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Plasmid; Strain; Sequence data
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200
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Ag-12
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Gene Synthesis
(151-1000bp)
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5-6
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Plasmid; Strain; Sequence data
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1.5/bp
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Ag-13
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Gene Synthesis (1001-2000bp)
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6-7
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Plasmid; Strain; Sequence data
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1.5/bp
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Ag-14
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Gene Synthesis (2001-4000bp)
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7-8
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Plasmid; Strain; Sequence data
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1.5/bp
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Ag-15
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Gene Synthesis (>4000bp)
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Inquire
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Plasmid; Strain; Sequence data
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1.5/bp
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Note: Default cloning vector is pGEM-T-easy vector; Please contact us if other vectors were needed.
Features: We are capable of synthesis a large number of genes.
Application: Generation of target genes.
The information required from customers:
Sequence of target genes;
Name of the vector.
Download order form
Gene Mutation
Gene mutation is a technique used to mutate target genes, including insertion, deletion and change of nucleotides.
Workflow:
List of services:
Catalog # |
Service
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Turn around time (W)
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Deliverables
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Prices (USD)
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Ag-16
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Point Mutation (One point, <1Kb)
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6
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Plasmid; Sequence data
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200
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Ag-17
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Point Mutation (One point, 1-2 Kb)
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8
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Plasmid; Sequence data
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300
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Ag-18
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Point Mutation (One point, >2Kb)
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Inquire
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Plasmid; Sequence data
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Inquire
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Ag-19
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Point Mutation (Two points, <1Kb)
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6
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Plasmid; Sequence data
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350
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Ag-20
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Point Mutation (Two points, 1-2 Kb)
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8
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Plasmid; Sequence data
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500
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Ag-21
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Point Mutation (Two points, >2Kb)
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Inquire
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Plasmid; Sequence data
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Inquire
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Note: Sequencing validation only for targeted region.
Features: One point mutation may contain 1-3 adjunction nucleotides alteration.
Application: Introducing DNA alteration, including changing open reading frame or amino acid sequence.
The information required from customers:
The plasmid containing target genes (>1µg)
Vector and target gene sequences
Desired mutations (please fill out the order form)
Bacterial stain contains plasmid
Download order form
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Info
